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Phototoxicity avoidance is a potential therapeutic approach for retinal dystrophy caused by EYS dysfunction

Otsuka Y, Imamura K, Oishi A, Asakawa K, Kondo T, Nakai R, Suga M, Inoue I, Sagara Y, Tsukita K, Teranaka K, Nishimura Y, Watanabe A, Umeyama K, Okushima N, Mitani K, Nagashima H, Kawakami K, Muguruma K, Tsujikawa A, Inoue H

JCI Insight (2024) 9(8):e174179


In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein.

Asakawa K*, Handa H, Kawakami K. (*Correspondence)

Methods in Molecular Biology (2024) 2707:257-264. doi: 10.1007/978-1-0716-3401-1_17.


Dysregulated TDP-43 proteostasis perturbs excitability of spinal motor neurons during brainstem-mediated fictive locomotion in zebrafish

Asakawa K*, Handa H, Kawakami K*.

Development, Growth & Differentiation (2023)  doi: 10.1007/978-1-0716-3401-1_17. (*Correspondence)


Optogenetic phase transition of TDP-43 in spinal motor neurons of zebrafish larvae

Asakawa K*, Handa H, Kawakami K.

Journal of Visualized Experiments (2022) doi: 10.3791/62932 (*Correspondence)


Illuminating ALS motor neurons with optogenetics in zebrafish

Asakawa K*, Handa H, Kawakami K.

Frontiers in Cell and Developmental Biology (2021) 9:640414 (*Correspondence)


Multi-phaseted problems of TDP-43 in selective neuronal vulnerability in ALS

Asakawa K*, Handa H, Kawakami K.

Cellular and Molecular Life Sciences (2021) 78(10):4453-4465 (*Correspondence)


Do not curse the darkness of the spinal cord, light TDP-43.

Asakawa K*, Handa H, Kawakami K.

Neural Regeneration Research (2021) 16:986-987 (*Correspondence)


Gsx2 is required for specification of neurons in the inferior olivary nuclei from Ptf1a-expressing neural progenitors in zebrafish.

Itoh T, Takeuchi M, Sakagami M, Asakawa K, Sumiyama K, Kawakami K, Shimizu T, Hibi M.

Development 147:dev190603 (2020)


Optogenetic modulation of TDP-43 oligomerization accelerates ALS-related pathologies in the spinal motor neurons.

Asakawa K*, Handa H, Kawakami K*.

Nature Communications 11:1004 (2020) (*Co-correspondence)


p63 is a cereblon substrate involved in thalidomide teratogenicity.

Asatsuma-Okumura T, Ando H, De Simone M, Yamamoto J, Sato T, Shimizu N, Asakawa K, Yamaguchi Y, Ito T, Guerrini L, Handa H.

Nature Chemical Biology 11:1077-1084 (2019)


Protocadherin-mediated cell repulsion controls the central topography and efferent projections of the abducens nucleus.

Asakawa K* and Kawakami K*.

Cell Reports 24:1562-1572 (2018) (*Co-correspondence)

#39 Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration. Ohki Y, Wenninger A, Hruscha A, Asakawa K, Kawakami K, Haass C, Edbauer D, Schmid B. Molecular Neurodegeneration 12:6 (2017) ​ #38 Visualization of Neuregulin 1 ectodomain shedding reveals its local processing in vitro and in vivo. Kamezaki A, Sato F, Aoki K, Asakawa K, Kawakami K, Matsuzaki F, Sehara-Fujisawa A. Scientific Reports 6:28873 (2016) ​ #37 Gal4 driver transgenic zebrafish: Powerful tools to study developmental biology, organogenesis and neuroscience. Kawakami K, Asakawa K, Hibi M, Ito M, Muto A, Wada H. Advances in Genetics 95:65-87 (2016) ​ #36 Tol2-mediated transgenesis, gene trapping, and enhancer trapping, and the Gal4-UAS system. Kawakami K, Asakawa K, Muto A, Wada H. Methods in Cell Biology 135:19-37 (2016) ​ #35 RING finger protein 121 facilitates the degradation and membrane localization of voltage-gated sodium channels. Ogino K, Low SE, Yamada K, Saint-Amant L, Zhou W, Muto A, Asakawa K, Nakai J, Kawakami K, Kuwada JY, Hirata H. Proc Natl Acad Sci U S A 112:2859-2864 (2015) ​ #34 Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry. Takeuchi M, Matsuda K, Yamaguchi S, Asakawa K, Miyasaka N, Lal P, Yoshihara Y, Koga A, Kawakami K, Shimizu T, Hibi M. Developmental Biology 397:1-17 (2015) ​ #33 The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish. Kwon HB, Fukuhara S, Asakawa K, Ando K, Kashiwada T, Kawakami K, Hibi M, Kwon YG, Kim KW, Alitalo K, Mochizuki N. Development 140:4081-4090 (2013) ​ #32 Wnt/Dkk negative feedback regulates sensory organ size in zebrafish. Wada H, Ghysen A, Asakawa K, Abe G, Ishitani T, Kawakami K. Current Biology 23:1559-1565 (2013) ​ #31 Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish. Asakawa K*, Gembu A and Kawakami K*. Frontiers in Neural Circuits 7:100 (2013) (*Co-correspondence) ​ #30 Interhemispheric asymmetry of olfactory input-dependent neuronal specification in the adult brain. Kishimoto N, Asakawa K, Madelaine R, Blader P, Kawakami K and Sawamoto K. Nature Neuroscience 16, 884-888 (2013) ​ #29 Glycinergic transmission and postsynaptic activation of CaMKII are required for glycine receptor clustering in vivo. Yamanaka I, Miki M, Asakawa K, Kawakami K, Oda Y and Hirata H. Genes to Cells 18:211-224 (2013) ​ #28 An mnr2b/hlxb9lb enhancer trap line that labels spinal and abducens motor neurons in zebrafish. Asakawa K, Higashijima S, and Kawakami K. Developmental Dynamics 241, 327-332 (2012) ​ #27 The ciliary protein Nek8/Nphp9 acts downstream of Inv/Nphp2 during pronephros morphogenesis and left-right establishment in zebrafish. Fukui H, Shiba D, Asakawa K, Kawakami K, and Yokoyama T. FEBS letters 586 (16), 2273-2279 (2012) ​ #26 Neuronal birth order identifies a dimorphic sensorineural map. Pujol-Marti J, Zecca A, Baudoin J.P, Faucherre A, Asakawa K, Kawakami K, and Lopez-Schier H. The Journal of Neuroscience 32, 2976-2987 (2012) ​ #25 Connexin 39.9 protein is necessary for coordinated activation of slow-twitch muscle and normal behavior in zebrafish. Hirata, H., Wen, H., Kawakami, Y., Naganawa, Y., Ogino, K., Yamada, K., Saint-Amant, L., Low, S.E., Cui, W.W., Zhou, W., Sprague, SM., Asakawa, K., Muto, A., Kawakami K., Kuwada JY. Journal of Biological Chemistry 287, 1080-1089 (2012) ​ #24 Migration of neuronal precursors from the telencephalic ventricular zone into the olfactory bulb in adult zebrafish. Kishimoto N, Alfaro-Cervello C, Shimizu K, Asakawa K, Urasaki A, Nonaka S, Kawakami K, Garcia-Verdugo JM, Sawamoto K. Journal of Comparative Neurology. 519, 3549-3565 (2012) ​ #23 zTrap: zebrafish gene trap and enhancer trap database. Kawakami K, Abe G, Asada T, Asakawa K, Fukuda R, Ito A, Lal P, Mouri N, Muto A, Suster ML, Takakubo H, Urasaki A, Wada H, Yoshida M. BMC Developmental Biology 10, 105 (2010) ​ #22 A transgenic zebrafish for monitoring in vivo microtubule structures. Asakawa K, Kawakami K. Developmental Dynamics 239, 2695-2699 (2010) ​ #21 Sex reversal in zebrafish fancl mutants is caused by Tp53-mediated germ cell apoptosis. Rodríguez-Marí A, Cañestro C, Bremiller RA, Nguyen-Johnson A, Asakawa K, Kawakami K, Postlethwait JH. PLoS Genetics. 6, e1001034 (2010) ​ #20 The Tol2-mediated Gal4-UAS method for gene and enhancer trapping in zebrafish. Asakawa K and Kawakami K. Methods 49:275-281 (2009) ​ #19 Olfactory Neural Circuitry for Attraction to Amino Acids Revealed by Transposon-Mediated Gene Trap Approach in zebrafish. Koide T, Miyasaka N, Morimoto K, Asakawa K, Urasaki A, Kawakami K, Yoshihara Y. Proc Natl Acad Sci U S A 106:9884-9889 (2009) ​ #18 Mechanism of development of ionocytes rich in vacuolar-type H+-ATPase in the skin of zebrafish larvae. Esaki M, Hoshijima K, Nakamura N, Munakata K, Tanaka M, Ookata K, Asakawa K, Kawakami K, Wang W, Weinberg ES, Hirose S. Developmental Biology 329:116-129 (2009) ​ #17 A novel conserved evx1 enhancer links spinal interneuron morphology and cis-regulation from fish to mammals. Suster ML, Kania A, Liao M, Asakawa K, Charron F, Kawakami K and Drapeau P. Developmental Biology 325: 422-433 (2009) ​ #16 Transgenesis in zebrafish with the Tol2 transposon system. Suster ML, Kikuta H, Urasaki A, Asakawa K and Kawakami K. Methods in Molecular Biology 561:41-63 (2009) ​ #15 Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish. Urasaki A, Asakawa K and Kawakami K. Proc Natl Acad Sci U S A 105: 19827-19832 (2008) ​ #14 Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish. Asakawa K, Suster ML, Mizusawa K, Nagayoshi S, Kotani T, Urasaki A, Kishimoto Y, Hibi M and Kawakami K. Proc Natl Acad Sci U S A 105:1255-1260 (2008) ​ #13 Insertional mutagenesis by the Tol2 transposon-mediated enhancer trap approach generated mutations in two developmental genes: tcf7 and synembryn-like. Nagayoshi S, Hayashi E, Abe G, Osato N, Asakawa K, Urasaki A, Horikawa K, Ikeo K, Takeda H and Kawakami K. Development 135:159-169 (2008) ​ #12 Targeted gene expression by the Gal4-UAS system in zebrafish. Asakawa K and Kawakami K. Development, Growth & Differentiation 50:391-399 (2008) ​ #11 Targeting neural circuitry in zebrafish using GAL4 enhancer trapping. Scott EK, Mason L, Arrenberg AB, Ziv L, Gosse JN, Xiao T, Chi NC, Asakawa K, Kawakami K and Baier H. Nature Methods 4: 323-326 (2007) ​ #10 Gamma-tubulin complex-mediated anchoring of spindle microtubules to spindle-pole bodies requires Msd1 in fission yeast. Toya M, Sato M, Haselmann U, Asakawa K, Brunner D, Antony C and Toda T. Nature Cell Biology 9:646-653 (2007) ​ #9 A rapid method for protein extraction from fission yeast. Matsuo Y, Asakawa K, Toda T and Katayama S. Bioscience Biotechnology and Biochemistry 70:1992-1994 (2006) ​ #8 The V260I Mutation in Fission Yeast α-Tubulin Atb2 Affects Microtubule Dynamics and EB1-Mal3 Localization and Activates the Bub1 Branch of the Spindle Checkpoint. Asakawa K*, Kume K*, Kanai M, Goshima T, Miyahara K, Dhut S, Tee WW, Hirata D and Toda T. Molecular Biology of the Cell 17: 1421-1435 (2006)(*co-first author) ​ #7 Cooperation of EB1-Mal3 and the Bub1 Spindle Checkpoint. Asakawa K and Toda T. Cell Cycle 5:27-30 (2006) ​ #6 Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment. Asakawa K, Toya M, Sato M, Kanai M, Kume K, Goshima T, Garcia MA, Hirata D and Toda T. EMBO Reports 6: 1194-1200 (2005) ​ #5 Inactivation of the pre-mRNA cleavage and polyadenylation factor Pfs2 in fission yeast causes lethal cell cycle defects. Wang SW*, Asakawa K*, Win TZ*, Toda, T and Norbury CJ. Molecular and Celluler Biology 25: 2288-2296 (2005) (*co-first author) ​ #4 A defect of Kap104 alleviates the requirement of MEN (mitotic exit network) gene functions in Saccharomyces cerevisiae. Asakawa K and Toh-e A. Genetics 162: 1545-1556 (2002) ​ #3 Mitotic Exit Network Controls the Localization of Cdc14 to the Spindle Pole Body in Saccharomyces cerevisiae. Yoshida S, Asakawa K, and Toh-e A. Current Biology 12:944-950 (2002) ​ #2 Spindle Checkpoint Control in Budding Yeast. Toh-e A, Asakawa K, and Yoshida S. The Journal of Microbiology 39, 1: 1-10 (2001) ​ #1 A novel functional domain of Cdc15 kinase is required for its interaction with Tem1GTPase in Saccharomyces cerevisiae. Asakawa K, Yoshida S, Otake F and Toh-e A. Genetics 157:1437-1450 (2001)

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